P-8: Evaluation of Protective Effect of Satureja Hortensis Extract against Cyclosporine-A Induced Changes in Testicular Tissue; A Histomorphometrical Analysis

Background: Cyclosporine-A (CsA) is an immunosuppressive drug used in organ transplantation. Side effects of CsA such as body weight loss and decreased fertility rate have been reported. Satureja hortensis is a therapeutic plant with antioxidant properties. Materials and Methods: 24 adult male rats weighing 200±20 gr were divided into three groups (n=8): control group which received no treatment; CsA group which received 40/mg/kg/day CsA and CsA-S. hortensis group received 40/mg/kg/day CsA and 100 mg/kg/day aqueous extract of S. hortensis with oral gavage. Half of each group was sacrificed on 21st day of treatment and the other half on 45th day, and the testicular tissues were collected and fixed and stained with Haematoxylin and Eosin (H&E). The prepared slides were evaluated with light microscope. Results: CsA significantly (p<0.01) decreased seminiferous tubules diameter, height of seminiferous epithelium (HSE) and number of Leydig cells compared to the control group. Furthermore, CsA increased number of mononuclear lymphatic cells in comparison with control group. S. hortensis in CsA-S. hortensis group partially improved the mentioned parameters. Conclusion: Considering the data, S. hortensis may ameliorate CsA induced detrimental effects on testis.